Furthermore, the possibility that JCV, which can cause demyelination in the brain, may also replicate in the brains of multiple sclerosis MS patients has not yet been completely excluded.
In addition, 58 centrifuged CSF samples 45 samples collected during an exacerbation and 13 samples collected during remission from 45 patients with early MS were also examined for the presence of JCV DNA.
CSF samples from 35 patients ages, 1 month to 67 years with aseptic meningitis were examined by virus isolation for the presence of enterovirus infection 6 , and 29 of these CSF samples were also examined by an enteroviral RNA-specific PCR 3 , 7.
Of the remaining 17 patients, whose CSF samples were negative by enterovirus isolation, 14 were tested with an enteroviral PCR. Two of these were enterovirus RNA positive, and 12 remained negative. The 45 MS patients ages, 21 to 55 years had laboratory-supported diagnoses of definite MS or clinically definite MS 16 and were rated according to an expanded disability status scale CSF samples were collected within 4 weeks after an exacerbation, defined as a sudden appearance of MS-related symptoms and signs or worsening of previous findings lasting more than 24 h.
None of the patients had been treated with immunosuppressive or antiviral drugs, including corticosteroids and beta interferon. The sensitivity of the PCR assay was 10 genome copies for both of the viruses. Furthermore, no JCV DNA could be detected in the CSF specimens from the 20 patients with confirmed enteroviral meningitis or from the 15 patients with meningitis of unknown etiology. However, this was not the case. Our results therefore indicate that inflammation in the brain observed during the conditions listed above does not necessarily cause reactivation of JCV.
Thus, in order to achieve an activation of JCV in the brain and to cause PML, immunosuppression alone or in combination with other unknown factors rather than simply an inflammation in the CNS is necessary. National Center for Biotechnology Information , U. Journal List J Clin Microbiol v. J Clin Microbiol. J Neurovirol ;9 Suppl Mult Scler Apr;12 2 Adv Exp Med Biol ; Clin Lab Med Dec;23 4 N Engl J Med Sep 10; 11 J Infect Dis Mar 15; 6 The LightCycler instrument Roche Applied Science amplifies and monitors the development of target nucleic acid sequences after the annealing step during PCR cycling.
This automated PCR system can rapidly detect amplicon development through stringent air-controlled temperature cycling in capillary cuvettes. The detection of amplified products is based on the fluorescence resonance energy transfer FRET principle.
For FRET product detection, a hybridization probe with a donor fluorophore, fluorescein, on the 3'-end is excited by an external light source and emits light that is absorbed by a second hybridization probe with an acceptor fluorophore, LC-Red , at the 5'-end.
The acceptor fluorophore then emits a light of a different wavelength that can be measured with a signal that is proportional to the amount of specific PCR product. Unpublished Mayo method. This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. Excel Pdf. Normal Reports Abnormal Reports.
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Includes If the Index Value is between 0. Service Area must be determined. Preferred Specimen s 1 mL serum. Room temperature: 7 days Refrigerated: 14 days Frozen: 90 days. Reference Values Negative. Day s Performed Monday through Friday. Test Classification This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. CPT Code Information Report Available 2 to 5 days.
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